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中南大学（学报）医学版 2010, 35(2) 100-106 DOI: 10.3969/j.issn.1672-7347.2010. ISSN: 1672-7347 CN: 43-1427/R

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	本文关键词相关文章
	降钙素基因相关肽
	髓样细胞触发受体-1
	脂多糖
	巨噬细胞
	本文作者相关文章
	PubMed

降钙素基因相关肽对脂多糖诱导的巨噬细胞
表达髓样细胞触发受体-1的影响

李云超1，管茶香2，周漾1，孙国瑛2，施萌1，邬晶1，李文杰1

中南大学湘雅医学院1.临床医学系； 2 生理学系，长沙 410078

摘要：

目的：探讨降钙素基因相关肽(CGRP)对脂多糖(LPS)诱导的小鼠巨噬细胞表达髓样细胞触发受体-1(TREM-1)的影响及其信号转导途径。方法：采用反转录-聚合酶链式反应（RT-PCR）观察巨噬细胞TREM-1 mRNA表达量的变化，应用流式细胞术检测巨噬细胞膜表面TREM-1蛋白的表达。结果：CGRP对未受刺激的巨噬细胞表达TREM-1无明显影响，LPS可诱导巨噬细胞表达TREM-1；CGRP预处理呈剂量和时间依赖性上调LPS所致的巨噬细胞TREM-1 mRNA的表达；同时，CGRP上调LPS所致的巨噬细胞膜表面TREM-1蛋白的表达；上述作用均可被PKC阻断剂H-7和PKA阻断剂H-89部分逆转(P<0.05)。结论：CGRP上调LPS诱导的巨噬细胞表达TREM-1，其胞内信号转导途径与PKA和PKC有关。

关键词：降钙素基因相关肽髓样细胞触发受体-1 脂多糖巨噬细胞

Effect of calcitonin gene-related peptide on the expression
of triggering receptor expressed on myeloid cells-1 in
lipopolysaccharide-induced macrophages

LI Yunchao1, GUAN Chaxiang2, ZHOU Yang1, SUN Guoying2, SHI Meng1, WU Jing1, LI Wenjie1

1.Department of Clinical Medicine; 2.Department of Physiology, Xiangya School of
Medicine, Central South University, Changsha 410078, China

Abstract:

ObjectiveTo determine the effect of calcitonin gene-related peptide (CGRP) on triggering receptor expressed on myeloid cells-1 (TREM-1) in the lipopolysaccharide (LPS)-induced macrophages and its signal transduction pathway. MethodsThe levels of TREM-1 mRNA in the macrophages were observed by reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry was performed to detect TREM-1 protein expression levels in the macrophages. ResultsCGRP had no regulating effect on the expression of TREM-1 in the macrophages; LPS could up-regulate macrophages to express TREM-1; CGRP increased TREM-1 mRNA expression in LPS-induced macrophages in dose and time-dependent manner; CGRP increased TREM-1 protein expression in LPS-induced macrophages, which could be partially reversed by H-7 or H-89 (P<0.05). ConclusionCGRP can regulate the LPS-induced macrophages synthesis and secretion of TREM-1, and the intracellular signal transduction pathway is related to PKA and PKC.

Keywords: calcitonin gene-related peptide;triggering receptor expressed on myeloid cells-1;lipopolysaccharide;macrophage

收稿日期 2009-11-10 修回日期 网络版发布日期

DOI: 10.3969/j.issn.1672-7347.2010.

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作者Email: gcxdzd91@163.com

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